Description
Aviva Systems Biology Pomc ELISA Kit (Rat) (OKCD02777) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Pomc antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Pomc and and incubated. The Pomc found in the sample or standards competes with the biotinylated Pomc for limited binding sites on the immobilized anti-Pomc antibody. Wells are washed and Avidin-HRP conjugate is added, incubated then washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Pomc captured in the well and inversely proportional to the amount of Pomc which was contained in the sample or standard